Transmission Electron Microscopy (TEM) is a specialized technique for the study of the ultrastructural detail of cells, tissues and organisms. TEM allows visual confirmation of specific subcellular structural abnormalities engendered by various diseases. The method also facilitates accurate identification of cell types, alterations in cell organelle structure and distribution, as well as characterization of abnormal cellular inclusions, to name a few. Information gleaned from TEM helps in refining histological diagnosis and predicting possible abnormalities in cell function. Any tissue/cell can be examined in this way, but results are optimized with tissue-specific preparation. At All Children’s hospital, TEM is utilized for many diseases including tumors, metabolic disorders and in-born errors of metabolism, neuromuscular diseases, renal disorders, infection (especially viral) diseases and others. In addition, biopsies for evaluating functional aspects of structures through the demonstration of ultrastructural abnormalities are also examined by TEM.
The laboratory is equipped with a JEOL 1210 Transmission Electron Microscope that is interfaced with the Internet to extend digital imaging capabilities of an AMT eleven Megapixel XR611/111 camera. Ultramicrotomes and tissue processors are employed for the sample preparation.
| Scientist & Unit Director | Peter Steele, Ph.D. Biological Sciences; Diplomate Laboratory Management, American Society for Clinical Pathology. Professional Member of the International Academy of Cytology & Society of Ultrastructural Pathology. |
| Transmission Electron Microscopy: | Monday-Friday 8:30 a.m. - 5:00 p.m. |
The charges for professional and technical services are institutional only, i.e., no third party billing can be performed.
Turn around time is dependent on the specimen type, complexity and associated histological evaluations if any. Interpretations are in the form of a surgical pathology report issued by the Attending Pathologist.
| Phone: | (727) 767-3058 (Histology Laboratory) |
| (727) 767-4465 (Scientist & Unit Director) | |
| Pager: | (727) 825-5131 |
| Fax: | (727) 767-8516 |
| E-mail: | Peter.Steele@allkids.org |
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