The PCRC Clinical Core includes research nursing and investigational pharmacy services.
The PCRC research nursing staff is comprised of skilled pediatric and neonatal registered nurses to assist principal investigators in the coordination and performance of scatterbed and outpatient studies.
Facilitating protocol implementing by reviewing for feasibility
Coordination of protocol implementation
Screening and identification of eligible subjects
Administration of research interventions
Assistance with procedures and specimen collection
Research record management
For additional information, please contact Dawn Bruton, Assistant Director of Nursing, 727-767-4617.
The Investigational Pharmacy was established to provide support for clinical drug research at All Children’s Hospital, and is located within the Inpatient Pharmacy. Services include the receiving, storing, dispensing and monitoring of investigational drugs to insure that all drug studies are conducted safely and in compliance with federal, state, JCAHO and IRB regulations. The Investigational Pharmacist will work directly with the investigator to facilitate accurate accountability of investigational drug products.
Sample Processing and Specimen Repository
The Sample Processing Laboratory and Specimen Repository Core Laboratories are located in approximately 1000 square feet of space on the fourth floor of the USF/ACH Children's Research Institute (CRI), in close proximity to the outpatient and inpatient services of the hospital. The necessary equipment for rapid specimen processing and storage, including centrifuges and biological safety cabinets are available. The Laboratory houses two biological safety cabinets, a floor model swinging bucket centrifuge, a cell analyzer, inverted microscope, standard microscope, refrigerator and freezer for storage of media and reagents, and a SANYO Model MCO-36AIC upright dual incubator for cell culture. An adjacent room houses four –85º Upright Freezers with CO2 backup, alarm, and long term cryopreservation system containing a -190º C liquid Nitrogen Storage tank capable of storing up to 36,000 individual samples. A third component of the laboratory, designed for the separation of the cellular components of human blood and assessments of cellular function, contains an Milyenyl Biotech Automax cell separator and ELISA plate reader and washer.
Biological Safety Level 3 Laboratory
The Biological Safety Level 3 Laboratory (BSL-3) is also located on the fourth floor of the CRI and meets current Centers for Disease Control guidelines. This space consists of approximately 400 square feet with an adjacent preparation room. It is equipped with air-flow systems for biological containment, and a biological safety seal entrance way, a dual access Autoclave/Sterilizer designed specifically for a BSL-3 laboratory, a bench top refrigerated centrifuge, two biological safety cabinets, a -85°C upright freezer with CO2 backup, a liquid nitrogen storage system, a double-bank incubator, an inverted microscope with digital camera, a microfuge, and PC computer system.
Located on the third floor of the CRI, this core provides high throughput genetic sequencing technology for automatic high resolution DNA sequencing. The equipment includes: an Applied Biosytems Gene Sequencing System 3730 with 48 Capillary capacity; and a CHEF Mapper XA Chiller System for pulsed field gel electrophoresis.
The Informatics Core provides access to hardware, software and data management services for investigators who are conducting research under approved PCRC protocols. The Core offers a secure infrastructure for data collection, storage and retrieval, backup and manipulation. The PCRC servers are available on the ACH campus network, which facilitates protected access, data storage and daily backups. The Informatics Core provides resources to:
- Provide analysis and planning of protocol informatics needs.
- Database design and implementation.
- Data translation services.
- Store, link, and merge clinical and outcomes data for patients enrolled in PCRC studies as well as national data sets (clinical and administrative) relevant to research on pediatric outcomes and quality (consistent with all applicable HIPPA regulations).
- Conduct outcomes research using selected Healthcare Costs and Utilization Project Data Set databases (National Inpatient Sample 1993-2004; State Inpatient Data 1990-2004; State Ambulatory Data 1997-2003; Kids Inpatient Data 1997, 2000, 2003; State Emergency Department Data 1997-2003); American Hospital Association Annual Survey 1996-2001, 2002, 2003, 2004; and 2003 Bureau of Health Professionals' Area Resource File.
Several computers and peripherals located in the PCRC Administrative Office are available to investigators including workstations, laptops, and color and black/white laser printers. For more information, please contact Sandy Wismer, 727-767-4813.